Key Responsibilities:
- Operation of experimental technology: follow SOP of PCR, glue recovery, etc. and complete the
delivery of PCR products - Follow the sequencing sheet or MES system and the previous handover, sort out and summarize the
schemes that need to be done on the day, and record the carriers and PCR products that need to be
prepared for their posts - Linearization of vectors and fragments: according to standard procedures, linearize the required
vectors and fragments with specific enzymes at specific temperatures and buffers - Connection and transformation: according to the experimental scheme, the products delivered by PCR
and the products prepared by employees should be matched with the linearized vector. After that,
splice into a ring through a specific connection method, and then introduce into specific competent
cells through heat shock. Then expand, culture and coat on a specific resistant plate - Record all data and results in specified forms (paper and electronic) with accuracy and responsibility
- Handover experiments properly, analyze the problems of uncompleted experiments, put forward
improvement plans, and report the uncompleted experiments to the supervisor - Maintain equipment and assist in ordering laboratory supplies
- Ensure that all safety guidelines are followed strictly at all times and maintain a clean and orderly
environment
Job Requirements:
- Bachelor's degree and above, with a certain understanding of microbiology and other biological-related
knowledge - Ability to work autonomously and under pressure
- Strong sense of responsibility and cost awareness, being proactive and creative
- Excellent professional ethics and team player