A research officer position is available immediately in the laboratory of Dr. Leslie Beh at IMCB.
The Beh lab seeks to develop cutting-edge CRISPR-Cas technologies to uncover the biological functions of RNA modifications. Dysregulation of these modifications has been linked to cancer, neurological disorders, and metabolic disease. We will determine the mechanisms by which RNA modifications and CRISPR-Cas systems operate by drawing upon diverse techniques, including protein engineering, biochemical reconstitution, genomics, and cell-based experiments. Successful applicants can expect close mentorship in a collaborative environment, with an emphasis on developing scientific knowledge and individual career goals. More information about can be found at (https://www.behlab.org/).
Requirements:
- B.Sc. (Hons) or Diploma in Life Sciences / Biomedical Sciences
- Interest in doing research and pursuing a research-based career.
- Experience in molecular biology techniques, including cloning, DNA and RNA purification, and protein expression / purification.
- Enthusiastic and skilled at solving difficult, multi-disciplinary problems.
- Strong work ethic, with excellent organizational and communication skills
Preferred experience:
- Recombinant protein purification from bacterial and insect cells
- Mammalian cell culture
- NGS library generation and data analysis
- Prior roles in lab management
Interested candidates could send a cover letter detailing your research motivations, CV, and the names and email addresses of three references to Dr. Leslie Beh ([email protected]).
Publications:
Hoffmann, F.*, Kim, M.*, Beh, L.Y.*, Wang, J., Vo, P.L.H., Gelsinger, D.R., Acree, C., Mohabir, J.T., Fernández, I.S., Sternberg, S.H. Selective recruitment of the AAA+ ATPase TnsC increases the fidelity of Type I-F CRISPR RNA-guided transposition. Nature (in press).
*Co-first author
Beh, L.Y., Debelouchina, G.T., Clay, D.M., Thompson, R.E., Lindblad, K.A., Hutton, E.R., Bracht, J.R., Sebra, R.P., Muir, T.W., Landweber, L.F. Identification of a DNA methyl-transferase complex and the impact of N6-methyladenine on chromatin organization.
Cell. 177(7):1781-96. Cover story
Beh, L.Y., Müller, M.M., Muir, T.W., Kaplan, N., Landweber, L.F. DNA-guided establishment of nucleosome patterns within coding regions of a eukaryotic genome. Genome Res. 25(11):1727-38.
Beh, L.Y., Colwell, L.J., Francis, N.J. A core subunit of Polycomb repressive complex 1 is broadly conserved in function but not primary sequence. Proc Natl Acad Sci U S A. 109: E1063-71.
Klompe, S.E., Jaber, N., Beh, L.Y., Mohabir, J.T., Sternberg, S.H. Evolutionary and mechanistic diversity of Type I-F CRISPR-associated transposons. Mol Cell. 82(3):616-628.
Feng, Y., Beh, L.Y. SIGAR: Inferring Features of Genome Architecture and DNA Rearrangements by Split-Read Mapping. Genome Biol Evol. 12(10):1711-18.
Wojcik, F., Dann, G.P., Beh, L.Y., Debelouchina, G.T., Hofmann, R., Muir, T.W. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants. Nat Commun. 9(1):1394.
Chen, X., Jung, S., Beh, L.Y., Eddy, S.R., Landweber, L.F. Combinatorial DNA rearrangement facilitates the origin of new genes in ciliates. Genome Biol Evol. 7(10):2859-70.
